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Highlights Monosodium glutamate aggravated sperm abnormalities of lipopolysaccharide-induced testicular lesions in rats, Monosodium glutamate exasperated hormonal imbalance in rats exposed to lipopolysaccharide, Monosodium glutamate exacerbated inflammation and oxidative stress in rats exposed to lipopolysaccharide, Weight deficit in monosodium glutamate-induced testicular lesion rats was worsened by lipopolysaccharide, Monosodium glutamate exacerbated the structural organisation of the testes in rats exposed to lipopolysaccharide